human glioblastoma a172 cell lines  (ATCC)

Journal: CNS Oncology

Article Title: Arginine deprivation induces prolonged autophagy activation and ROS-dependent cell death in glioblastoma multiforme

doi: 10.1080/20450907.2026.2666026

Figure Lengend Snippet: Cytotoxicity of GBM cell lines A172 and U251 following prolonged arginine deprivation (A). Average ASS-1 levels, expressed as mean fluorescence intensity (MFI), for each condition (isotype, untreated and treated cells) at 24, 48, 72, 96, and 120 hours post-treatment. Stars indicate a significant increase in ASS-1 expression compared to both isotype control and untreated cells (B) ASS-1 expression of A172 and U251 cell lines at 72 hours post-treatment. MFI is on the X-axis (FL1-H) and forward scatter is on the Y-axis (C).

Article Snippet: Human Glioblastoma cell lines A172 and U251 MG were obtained from the American Type Culture Collection (A172 ATCC, CRL-1620) and from the European Collection of Authenticated Cell Cultures (U251 MG, ECACC, catalog # 09063001).

Techniques: Fluorescence, Expressing, Control

Journal: CNS Oncology

Article Title: Arginine deprivation induces prolonged autophagy activation and ROS-dependent cell death in glioblastoma multiforme

doi: 10.1080/20450907.2026.2666026

Figure Lengend Snippet: Autophagosome accumulation in GBM cells, expressed as mean fluorescence intensity (MFI), for control untreated cells and cells treated with [HuArgI (Co)-PEG5000] at 24, 48, 72, 96, and 120 hours post-treatment. Stars indicate a significant increase in autophagosome accumulation compared to untreated cells (A). Autophagosome accumulation in A172 and U251 cell lines at 72 hours post-treatment in untreated cells and cells treated with CQ, [HuArgI (Co)-PEG5000] and a combination of both. MFI is on the X-axis (FL1-H) and forward scatter is on the Y-axis (B). Immunohistochemistry staining of autophagosomes in A172 cells untreated and treated with CQ, [HuArgI (Co)-PEG5000], or a combination of both, at 24, 48, and 72 hours, post-treatment (C).

Article Snippet: Human Glioblastoma cell lines A172 and U251 MG were obtained from the American Type Culture Collection (A172 ATCC, CRL-1620) and from the European Collection of Authenticated Cell Cultures (U251 MG, ECACC, catalog # 09063001).

Techniques: Fluorescence, Control, Immunohistochemistry, Staining

Journal: CNS Oncology

Article Title: Arginine deprivation induces prolonged autophagy activation and ROS-dependent cell death in glioblastoma multiforme

doi: 10.1080/20450907.2026.2666026

Figure Lengend Snippet: Western blot of Atg13 and phosphor-Atg13 on protein extracts of A172 and U251 untreated and treated with [HuArgI (Co)-PEG5000] for up to 96 hours (Atg13) (A). Histogram of the ratio of p-Atg13 over Atg13 showing a significant decrease in the phosphorylation of Atg13 at 72- and 96-hours post-treatment. Stars indicate statistical significance (A). ULK phosphorylation levels expressed as mean fluorescence intensity (MFI), for untreated cells and cells treated with [HuArgI (Co)-PEG5000] at 24, 48, 72, 96, and 120 hours post-treatment. Double stars indicate significantly lower ULK phosphorylation in treated compared to untreated cells (B). ULK phosphorylation is also shown in A172 and U251 cell lines at 24- and 48-hours post-treatment in isotype controls, untreated cells and cells treated with [HuArgI (Co)-PEG5000]. MFI is represented on the X-axis (FL1-H) and forward scatter is represented on the Y-axis. Last panel on the right is a histogram representing the overlayed phospho-ULK MFI of the isotype control (red), untreated cells (black) and cells treated with [HuArgI (Co)-PEG5000] (blue) (B). Cytotoxicity of [HuArgI (Co)-PEG5000] alone and in combination with chloroquine (CQ) (50 µM) to A172 and U251 cells at 48, 72, 96, and 120 hours (C).

Article Snippet: Human Glioblastoma cell lines A172 and U251 MG were obtained from the American Type Culture Collection (A172 ATCC, CRL-1620) and from the European Collection of Authenticated Cell Cultures (U251 MG, ECACC, catalog # 09063001).

Techniques: Western Blot, Phospho-proteomics, Fluorescence, Control

Journal: CNS Oncology

Article Title: Arginine deprivation induces prolonged autophagy activation and ROS-dependent cell death in glioblastoma multiforme

doi: 10.1080/20450907.2026.2666026

Figure Lengend Snippet: Intracellular ROS accumulation in GBM cells following treatment with [HuArgI (Co)|-PEG5000], expressed as both percent positive cells (left panel) and mean fluorescence intensity (MFI) (right panel). Stars indicate a significant increase in ROS accumulation. The bottom histograms show ROS accumulation in treated cells (red) compared to controls (black) in A172 and U251 cells. (A) Autophagosome accumulation in GBM cells, expressed as MFI, for control untreated cells and cells treated with [HuArgI (Co)-PEG5000] alone or a combination of [HuArgI (Co)-PEG5000] and N-acetylcysteine (NAC) at 24, 48, 72, 96, and 120 hours post-treatment (B). Autophagosome formation in A172 and U251 cell lines. Control untreated cells (left panel) and cells treated with [HuArgI (Co)-PEG5000] (10 −7 M) (middle panel) or treated with [HuArgI (Co)-PEG5000] and NAC (0.82 g/L) (right panel) at 24, 48, 72, 96, and 120-hours post-treatment. In the histogram (right panel), control cells are in black, cells treated with [HuArgI (Co)-PEG5000] are in red and cells treated with the combination with NAC are in blue (C). Cytotoxicity of A172 and U251 cells treated with [HuArgI (Co)-PEG5000] alone or in combination with N-acetylcysteine (NAC) (0.82 g/L) (D).

Article Snippet: Human Glioblastoma cell lines A172 and U251 MG were obtained from the American Type Culture Collection (A172 ATCC, CRL-1620) and from the European Collection of Authenticated Cell Cultures (U251 MG, ECACC, catalog # 09063001).

Techniques: Fluorescence, Control